Perampanel Reduces Brain Damage via Induction of M2 Microglia in a Neonatal Rat Stroke Model.
Int J Nanomedicine
; 17: 2791-2804, 2022.
Article
in En
| MEDLINE
| ID: mdl-35782016
ABSTRACT
Purpose:
Ischemic stroke is a leading cause of death and disability worldwide. Additionally, neonatal ischemia is a common cause of neonatal brain injury, resulting in cerebral palsy with subsequent learning disabilities and epilepsy. However, there is currently a lack of effective treatments available for patients with perinatal ischemic stroke. In this study, we investigated the effect of perampanel (PER)-loaded poly lactic-co-glycolic acid (PLGA) by targeting microglia in perinatal stroke.Methods:
After formation of focal ischemic stroke by photothrombosis in P7 rats, PER-loaded PLGA was injected intrathecally. Proinflammatory markers (TNF-α, IL-1ß, IL-6, COX2, and iNOS) and M2 polarization markers (Ym1 and Arg1) were evaluated. We investigated whether PER increased M2 microglial polarization in vitro.Results:
PER-loaded PLGA nanoparticles decreased the pro-inflammatory cytokines compared to the control group. Furthermore, they increased M2 polarization.Conclusion:
PER-loaded PLGA nanoparticles decreased the size of the infarct and increased motor function in a perinatal ischemic stroke rat model. Pro-inflammatory cytokines were also reduced compared to the control group. Finally, this development of a drug delivery system targeting microglia confirms the potential to develop new therapeutic agents for perinatal ischemic stroke.Key words
Full text:
1
Database:
MEDLINE
Main subject:
Brain Injuries
/
Brain Ischemia
/
Stroke
/
Ischemic Stroke
Limits:
Animals
Language:
En
Year:
2022
Type:
Article