Detection of Chemokine Binding Proteins Association to Cell Surface Glycosaminoglycans by Flow Cell Cytometry and Indirect Immunofluorescence.
Methods Mol Biol
; 2597: 121-129, 2023.
Article
in En
| MEDLINE
| ID: mdl-36374418
ABSTRACT
Viruses encode secreted proteins that bind chemokines to modulate their activity. Viral proteins may simultaneously interact with glycosaminoglycans allowing these proteins to be anchored at the cell surface to increase their anti-chemokine activity in the proximity of infection. Here we describe methodology to evaluate the interaction of viral secreted proteins with cell-surface glycosaminoglycans by immunofluorescence and detection by flow cytometry or microscopy. These methods could be equally applied to other chemokine binding proteins that do not have viral origin.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Carrier Proteins
/
Glycosaminoglycans
Language:
En
Year:
2023
Type:
Article