Molecular mechanism of di-n-butyl phthalate promotion of bladder cancer development.
Toxicol In Vitro
; 86: 105508, 2023 Feb.
Article
in En
| MEDLINE
| ID: mdl-36375677
ABSTRACT
PURPOSE:
To determine whether di-n-butyl phthalate (DBP) promotes the occurrence of bladder cancer (BCa) and explore the action of DBP acts on BCa cells at the cellular and molecular levels.METHODS:
MTT and Transwell assays were used to investigate the tumorigenic actions of DBP on BCa cells. Second-generation sequencing was used to identify differences in gene expression before and after DBP treatment. Differential gene expression was verified by q-PCR and analyzed using bioinformatics. Cells were transfected to overexpress genes of interest and proliferation and migration were measured using MTT and Transwell assays, respectively.RESULTS:
DBP treatment stimulated both proliferation and invasion in BCa cells. Second-generation sequencing identified differences in the expression of FOSB, JUND, ATP6V1C2, and RHOQ before and after DBP treatment. FOSB expression was confirmed by q-PCR and bioinformatic analyses. FOSB overexpression increased both proliferation and invasion in BCa cells.CONCLUSION:
DBP promoted BCa tumorigenesis by inducing changes in gene expression.Key words
Full text:
1
Database:
MEDLINE
Main subject:
Urinary Bladder Neoplasms
/
Dibutyl Phthalate
Limits:
Humans
Language:
En
Year:
2023
Type:
Article