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Detection of Streptococcus pyogenes M1UK in Australia and characterization of the mutation driving enhanced expression of superantigen SpeA.
Davies, Mark R; Keller, Nadia; Brouwer, Stephan; Jespersen, Magnus G; Cork, Amanda J; Hayes, Andrew J; Pitt, Miranda E; De Oliveira, David M P; Harbison-Price, Nichaela; Bertolla, Olivia M; Mediati, Daniel G; Curren, Bodie F; Taiaroa, George; Lacey, Jake A; Smith, Helen V; Fang, Ning-Xia; Coin, Lachlan J M; Stevens, Kerrie; Tong, Steven Y C; Sanderson-Smith, Martina; Tree, Jai J; Irwin, Adam D; Grimwood, Keith; Howden, Benjamin P; Jennison, Amy V; Walker, Mark J.
Affiliation
  • Davies MR; Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, Australia. mark.davies1@unimelb.edu.au.
  • Keller N; Australian Infectious Diseases Research Centre and School of Chemistry and Molecular Biosciences and Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD, Australia.
  • Brouwer S; Australian Infectious Diseases Research Centre and School of Chemistry and Molecular Biosciences and Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD, Australia.
  • Jespersen MG; Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, Australia.
  • Cork AJ; Australian Infectious Diseases Research Centre and School of Chemistry and Molecular Biosciences and Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD, Australia.
  • Hayes AJ; Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, Australia.
  • Pitt ME; Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, Australia.
  • De Oliveira DMP; Australian Infectious Diseases Research Centre and School of Chemistry and Molecular Biosciences and Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD, Australia.
  • Harbison-Price N; Australian Infectious Diseases Research Centre and School of Chemistry and Molecular Biosciences and Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD, Australia.
  • Bertolla OM; Australian Infectious Diseases Research Centre and School of Chemistry and Molecular Biosciences and Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD, Australia.
  • Mediati DG; School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, Australia.
  • Curren BF; Australian Infectious Diseases Research Centre and School of Chemistry and Molecular Biosciences and Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD, Australia.
  • Taiaroa G; Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, Australia.
  • Lacey JA; Department of Infectious Diseases, The University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, Australia.
  • Smith HV; Public Health Microbiology, Queensland Health Forensic and Scientific Services, Queensland Health, Coopers Plains, QLD, Australia.
  • Fang NX; Public Health Microbiology, Queensland Health Forensic and Scientific Services, Queensland Health, Coopers Plains, QLD, Australia.
  • Coin LJM; Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, Australia.
  • Stevens K; Microbiological Diagnostic Unit Public Health Laboratory, The Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, Australia.
  • Tong SYC; Department of Infectious Diseases, The University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, Australia.
  • Sanderson-Smith M; Victorian Infectious Diseases Service, The Royal Melbourne Hospital, at the Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, Australia.
  • Tree JJ; Illawarra Health and Medical Research Institute and Molecular Horizons, School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW, Australia.
  • Irwin AD; School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, Australia.
  • Grimwood K; University of Queensland Centre for Clinical Research, Brisbane, QLD, Australia.
  • Howden BP; Queensland Children's Hospital, Brisbane, QLD, Australia.
  • Jennison AV; School of Medicine and Dentistry and Menzies Health Institute Queensland, Griffith University, Gold Coast, QLD, Australia.
  • Walker MJ; Departments of Infectious Diseases and Paediatrics, Gold Coast Health, Gold Coast, QLD, Australia.
Nat Commun ; 14(1): 1051, 2023 02 24.
Article in En | MEDLINE | ID: mdl-36828918
ABSTRACT
A new variant of Streptococcus pyogenes serotype M1 (designated 'M1UK') has been reported in the United Kingdom, linked with seasonal scarlet fever surges, marked increase in invasive infections, and exhibiting enhanced expression of the superantigen SpeA. The progenitor S. pyogenes 'M1global' and M1UK clones can be differentiated by 27 SNPs and 4 indels, yet the mechanism for speA upregulation is unknown. Here we investigate the previously unappreciated expansion of M1UK in Australia, now isolated from the majority of serious infections caused by serotype M1 S. pyogenes. M1UK sub-lineages circulating in Australia also contain a novel toxin repertoire associated with epidemic scarlet fever causing S. pyogenes in Asia. A single SNP in the 5' transcriptional leader sequence of the transfer-messenger RNA gene ssrA drives enhanced SpeA superantigen expression as a result of ssrA terminator read-through in the M1UK lineage. This represents a previously unappreciated mechanism of toxin expression and urges enhanced international surveillance.
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Full text: 1 Database: MEDLINE Main subject: Scarlet Fever / Streptococcal Infections Type of study: Diagnostic_studies Limits: Humans Country/Region as subject: Europa / Oceania Language: En Year: 2023 Type: Article
Fulltext
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Full text: 1 Database: MEDLINE Main subject: Scarlet Fever / Streptococcal Infections Type of study: Diagnostic_studies Limits: Humans Country/Region as subject: Europa / Oceania Language: En Year: 2023 Type: Article