Human serum albumin-based supramolecular host-guest boronate probe for enhanced peroxynitrite sensing.
Org Biomol Chem
; 21(22): 4661-4666, 2023 06 07.
Article
in En
| MEDLINE
| ID: mdl-37212349
ABSTRACT
Peroxynitrite (ONOO-) is an important oxygen/nitrogen reactive species implicated in a number of physiological and pathological processes. However, due to the complexity of the cellular micro-environment, the sensitive and accurate detection of ONOO- remains a challenging task. Here, we developed a long-wavelength fluorescent probe based on the conjugation between a TCF scaffold and phenylboronate; the resulting conjugate is capable of supramolecular host-guest assembly with human serum albumin (HSA) for the fluorogenic sensing of ONOO-. The probe exhibited an enhanced fluorescence over a low concentration range of ONOO- (0-9.6 µM), whist the fluorescence was quenched when the concentration of ONOO- exceeded 9.6 µM. In addition, when human serum albumin (HSA) was added, the initial fluorescence of the probe was significantly enhanced, which enabled the more sensitive detection of low-concentrations of ONOO- in aqueous buffer solution and in cells. The molecular structure of the supramolecular host-guest ensemble was determined using small-angle X-ray scattering.
Full text:
1
Database:
MEDLINE
Main subject:
Peroxynitrous Acid
/
Fluorescent Dyes
Limits:
Humans
Language:
En
Year:
2023
Type:
Article