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High-resolution genome mapping and functional dissection of chlorogenic acid production in Lonicera maackii.
Li, Ruili; Xu, Jing; Qi, Zengxing; Zhao, Shiwei; Zhao, Ran; Ge, Yanrui; Li, Ruofan; Kong, Xiuya; Wu, Zhenying; Zhang, Xi; He, Qizouhong; Zhang, Yan; Liu, Ping-Li; Zhu, Lei; Mao, Jian-Feng; Fu, Chunxiang; Komis, George; Grünhofer, Paul; Schreiber, Lukas; Lin, Jinxing.
Affiliation
  • Li R; State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
  • Xu J; National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
  • Qi Z; Institute of Tree Development and Genome Editing, Beijing Forestry University, Beijing 100083, China.
  • Zhao S; State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
  • Zhao R; National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
  • Ge Y; Institute of Tree Development and Genome Editing, Beijing Forestry University, Beijing 100083, China.
  • Li R; State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
  • Kong X; National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
  • Wu Z; Institute of Tree Development and Genome Editing, Beijing Forestry University, Beijing 100083, China.
  • Zhang X; State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
  • He Q; National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
  • Zhang Y; Institute of Tree Development and Genome Editing, Beijing Forestry University, Beijing 100083, China.
  • Liu PL; State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
  • Zhu L; National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
  • Mao JF; Institute of Tree Development and Genome Editing, Beijing Forestry University, Beijing 100083, China.
  • Fu C; State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
  • Komis G; National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
  • Grünhofer P; Institute of Tree Development and Genome Editing, Beijing Forestry University, Beijing 100083, China.
  • Schreiber L; State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
  • Lin J; National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
Plant Physiol ; 192(4): 2902-2922, 2023 08 03.
Article in En | MEDLINE | ID: mdl-37226859
ABSTRACT
Amur honeysuckle (Lonicera maackii) is a widely used medicinal plant of the Caprifoliaceae family that produces chlorogenic acid. Research on this plant mainly focuses on its ornamental value and medicinal compounds, but a reference genome sequence and molecular resources for accelerated breeding are currently lacking. Herein, nanopore sequencing and high-throughput chromosome conformation capture (Hi-C) allowed a chromosome-level genome assembly of L. maackii (2n = 18). A global view of the gene regulatory network involved in the biosynthesis of chlorogenic acid and the dynamics of fruit coloration in L. maackii was established through metabolite profiling and transcriptome analyses. Moreover, we identified the genes encoding hydroxycinnamoyl-CoA quinate transferase (LmHQT) and hydroxycinnamoyl-CoA shikimic/quinate transferase (LmHCT), which localized to the cytosol and nucleus. Heterologous overexpression of these genes in Nicotiana benthamiana leaves resulted in elevated chlorogenic acid contents. Importantly, HPLC analyses revealed that LmHCT and LmHQTs recombinant proteins modulate the accumulation of chlorogenic acid (CGA) using quinic acid and caffeoyl CoA as substrates, highlighting the importance of LmHQT and LmHCT in CGA biosynthesis. These results confirmed that LmHQTs and LmHCT catalyze the biosynthesis of CGA in vitro. The genomic data presented in this study will offer a valuable resource for the elucidation of CGA biosynthesis and facilitating selective molecular breeding.
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Full text: 1 Database: MEDLINE Main subject: Chlorogenic Acid / Lonicera Language: En Year: 2023 Type: Article
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Full text: 1 Database: MEDLINE Main subject: Chlorogenic Acid / Lonicera Language: En Year: 2023 Type: Article