Combined Analysis of mRNA Expression and Open Chromatin in Microglia.
Methods Mol Biol
; 2713: 543-571, 2024.
Article
in En
| MEDLINE
| ID: mdl-37639146
ABSTRACT
The advance of single-cell RNA-sequencing technologies in the past years has enabled unprecedented insights into the complexity and heterogeneity of microglial cell states in the homeostatic and diseased brain. This includes rather complex proteomic, metabolomic, morphological, transcriptomic, and epigenetic adaptations to external stimuli and challenges resulting in a novel concept of core microglia properties and functions. To uncover the regulatory programs facilitating the rapid transcriptomic adaptation in response to changes in the local microenvironment, the accessibility of gene bodies and gene regulatory elements can be assessed. Here, we describe the application of a previously published method for simultaneous high-throughput ATAC and RNA expression with sequencing (SHARE-seq) on microglia nuclei isolated from frozen mouse brain tissue.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Chromatin
/
Microglia
Limits:
Animals
Language:
En
Year:
2024
Type:
Article