Hypersecretory production of glucose oxidase in Pichia pastoris through combinatorial engineering of protein properties, synthesis, and secretion.

Zhou, Huzhi; Zhang, Wenyu; Qian, Jiangchao

Zhou, Huzhi; Zhang, Wenyu; Qian, Jiangchao.

Affiliation

Zhou H; School of Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Zhang W; School of Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Qian J; School of Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.

Biotechnol Bioeng ; 121(2): 735-748, 2024 Feb.

Article in En | MEDLINE | ID: mdl-38037762

Subject(s)

Glucose Oxidase; Pichia; Saccharomycetales; Glucose Oxidase/genetics; Glucose Oxidase/metabolism; Pichia/metabolism; Bioreactors; Fermentation; Recombinant Proteins/genetics; Recombinant Proteins/metabolism

Key words

Pichia pastoris; glucose oxidase; saturation mutagenesis; secretory production; signal peptide; vesicle trafficking

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Full text: 1 Database: MEDLINE Main subject: Pichia / Saccharomycetales / Glucose Oxidase Language: En Year: 2024 Type: Article

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Full text: 1 Database: MEDLINE Main subject: Pichia / Saccharomycetales / Glucose Oxidase Language: En Year: 2024 Type: Article