ABSTRACT
Introduction:
Bisphenol A (BPA) is a substance
belonging to the endocrine-disrupting
chemicals, globally used in the
production of polycarbonate
plastics. It has been found that BPA enhances
carcinogenesis, triggers
obesity and exerts a pathogenic effect in several disorders, such as
type 2 diabetes,
asthma, or increased
blood pressure. Recent studies have revealed, that BPA has a harmful impact on the
kidneys function, therefore, the current
research aimed to explore the specific molecular changes triggered in these organs after oral BPA exposure in
mice. Materials and
Methods:
The experiment was carried out on 12 (3-month-old)
female mice. Six
mice served as controls. The other 6
mice were treated with BPA in the
drinking water at a
dose of 50 mg/kg b. w. for 3 months. Then
animals were euthanized, the
kidneys were collected, and extracted
RNA was used to perform
RNA-seq.
Results:
Applied multistep
bioinformatics revealed 433 differentially expressed
genes (DEGs) in the BPA-treated
kidneys (232 upregulated and 201 downregulated). Additionally, 95 differentially expressed long-noncoding RNAs (DELs) were revealed in BPA samples. The
Gene Ontology (GO) and Kyoto
Encyclopedia of
Genes and
Genomes (KEGG) annotations indicated that BPA exposure resulted in profound changes in several essential processes, such as
oxidative phosphorylation, mitochondrial and
ribosome function, or chemical
carcinogenesis.
Conclusion:
The obtained novel results suggest that BPA has a harmful impact on the fundamental processes of the
kidney and significantly impairs its function by inducing
mitochondrial dysfunction leading to
oxidative stress and
reactive oxygen species production.
