An optimized protocol for isolation of murine pancreatic single cells with high yield and purity.
STAR Protoc
; 5(1): 102836, 2024 Mar 15.
Article
in En
| MEDLINE
| ID: mdl-38219150
ABSTRACT
Here, we present a protocol for rapidly isolating single cells from the mouse pancreas, minimizing damage caused by digestive enzymes in exocrine cells. We guide you through steps to optimize the dissection sequence, enzyme composition, and operational procedures, resulting in high yields of viable pancreatic single cells. This protocol can be applied across a wide range of research areas, including single-cell sequencing, gene expression profiling, primary cell culture, and even the development of spheroids or organoids. For complete details on the use and execution of this protocol, please refer to Jiang et al. (2023).1.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Pancreas
/
Pancreatic Hormones
Limits:
Animals
Language:
En
Year:
2024
Type:
Article