A web-based dashboard for RELION metadata visualization.
Acta Crystallogr D Struct Biol
; 80(Pt 2): 93-100, 2024 Feb 01.
Article
in En
| MEDLINE
| ID: mdl-38265874
ABSTRACT
Cryo-electron microscopy (cryo-EM) has witnessed radical progress in the past decade, driven by developments in hardware and software. While current software packages include processing pipelines that simplify the image-processing workflow, they do not prioritize the in-depth analysis of crucial metadata, limiting troubleshooting for challenging data sets. The widely used RELION software package lacks a graphical native representation of the underlying metadata. Here, two web-based tools are introduced relion_live.py, which offers real-time feedback on data collection, aiding swift decision-making during data acquisition, and relion_analyse.py, a graphical interface to represent RELION projects by plotting essential metadata including interactive data filtration and analysis. A useful script for estimating ice thickness and data quality during movie pre-processing is also presented. These tools empower researchers to analyse data efficiently and allow informed decisions during data collection and processing.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Image Processing, Computer-Assisted
/
Metadata
Language:
En
Year:
2024
Type:
Article