Terminal regions of UAP56 and URH49 are required for their distinct complex formation functioning to an essential role in mRNA processing and export.
Biochem Biophys Res Commun
; 703: 149682, 2024 04 09.
Article
in En
| MEDLINE
| ID: mdl-38377942
ABSTRACT
UAP56 and URH49 are closely related RNA helicases that function in selective mRNA processing and export pathways to fine-tune gene expression through distinct complex formations. The complex formation of UAP56 and URH49 is believed to play a crucial role in regulating target mRNAs. However, the mechanisms underlying this complex formation have not been fully elucidated. Here we identified the regions essential for the complex formation of both helicases. The terminal regions of UAP56 and the C-terminal region of URH49 were indispensable for their respective complex formation. Further analysis revealed that a specific amino acid at the C-terminus of UAP56 is critical for its complex formation. Alanine substitution of this amino acid impairs its complex formation and subsequently affected its mRNA processing and export activity. Our study provides a deeper understanding of the basis for the complex formation between UAP56 and URH49.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
RNA Processing, Post-Transcriptional
/
RNA Helicases
/
DEAD-box RNA Helicases
Limits:
Humans
Language:
En
Year:
2024
Type:
Article