Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell function.
Sci Signal
; 17(827): eade0580, 2024 Mar 12.
Article
in En
| MEDLINE
| ID: mdl-38470957
ABSTRACT
Intercellular communication between different cell types in solid tumors contributes to tumor growth and metastatic dissemination. The secretome of cancer-associated fibroblasts (CAFs) plays major roles in these processes. Using human mammary CAFs, we showed that CAFs with a myofibroblast phenotype released extracellular vesicles that transferred proteins to endothelial cells (ECs) that affected their interaction with immune cells. Mass spectrometry-based proteomics identified proteins transferred from CAFs to ECs, which included plasma membrane receptors. Using THY1 as an example of a transferred plasma membrane-bound protein, we showed that CAF-derived proteins increased the adhesion of a monocyte cell line to ECs. CAFs produced high amounts of matrix-bound EVs, which were the primary vehicles of protein transfer. Hence, our work paves the way for future studies that investigate how CAF-derived matrix-bound EVs influence tumor pathology by regulating the function of neighboring cancer, stromal, and immune cells.
Full text:
1
Database:
MEDLINE
Main subject:
Cancer-Associated Fibroblasts
/
Neoplasms
Limits:
Humans
Language:
En
Year:
2024
Type:
Article