Protocol to encapsulate cerebral organoids with alginate hydrogel shell to induce volumetric compression.
STAR Protoc
; 5(2): 102952, 2024 Jun 21.
Article
in En
| MEDLINE
| ID: mdl-38555589
ABSTRACT
In vitro organoids, including cerebral organoids, are usually developed without mechanical compression, which may contribute to a delay in maturation. Here, we present a protocol for encapsulating cerebral organoids with a thin shell of low-concentration alginate hydrogel. We describe steps for organoid generation, microfluidic chip culture, Matrigel coating, expansion culture, and alginate encapsulation. We then detail procedures for maturation culture and organoid characterization. The moderate compressive stimulation that the shell provides promotes cell proliferation and neuronal maturation. For complete details on the use and execution of this protocol, please refer to Tang et al.1.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Organoids
/
Hydrogels
/
Alginates
Limits:
Animals
/
Humans
Language:
En
Year:
2024
Type:
Article