On-Chip Magnetic Extraction of Circulating Cell-Free DNA from Biological Samples.
Methods Mol Biol
; 2804: 65-75, 2024.
Article
in En
| MEDLINE
| ID: mdl-38753140
ABSTRACT
In recent years, the analysis of circulating cell-free DNA (cfDNA) containing tumor-derived DNA has emerged as a noninvasive means for cancer monitoring and personalized medicine. However, the isolation of cfDNA from peripheral blood has remained a challenge due to the low abundance and high fragmentation of these molecules. Here, we present a dynamic Magnetic ExTRactiOn (METRO) protocol using microfluidic fluidized bed technology to isolate circulating cfDNA from raw biological materials such as undiluted serum. This protocol maximizes the surface area for DNA binding within the chip in order to capture short DNA fragments. It uses only a few µL of sample and reagents. The protocol can be automated, and it is fully compatible with sensitive DNA amplification methods such as droplet-based digital PCR (ddPCR).
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Lab-On-A-Chip Devices
/
Cell-Free Nucleic Acids
Limits:
Humans
Language:
En
Year:
2024
Type:
Article