ABSTRACT
Purpose:
This study investigated the differences in the maturation rate of single versus grouped
cumulus-
oocyte complexes (COCs)
culture methods for capacitation in vitro maturation (CAPA-IVM) in
women with
polycystic ovary syndrome (PCOS).
Methods:
This study was performed at My Duc Phu Nhuan
Hospital,
Vietnam from October 1, 2020 to October 24, 2021.
Women aged 18-37 years with a
diagnosis of PCOS were recruited. COCs from each
woman were randomly divided into two groups single or grouped
culture during CAPA-IVM
culture. The primary outcome was the maturation rate.
Results:
A total of 322 COCs from 15 eligible
women included were randomly assigned to the two study groups. The maturation rate was comparable between the single and grouped
culture groups (61.3% vs. 64.8%; p = 0.56). There were no significant differences in the number of 2-pronuclei fertilized
oocytes, number of day-3
embryos, and number of good-quality
embryos in the two
culture method groups. In the single
culture group, COCs morphology was associated with the day-3
embryo formation rate but not the maturation rate.
Conclusions:
Comparable
oocyte maturation and
embryology outcomes between single and grouped COCs
culture utilizing
sibling COCs derived from
women with PCOS suggest the feasibility of both
methods for CAPA-IVM
culture.
