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Time-course transcriptome data of silk glands in day 0-7 last-instar larvae of Bombyx mori (w1 pnd strain).
Masuoka, Yudai; Jouraku, Akiya; Tsubota, Takuya; Ono, Hiromasa; Chiba, Hirokazu; Sezutsu, Hideki; Bono, Hidemasa; Yokoi, Kakeru.
Affiliation
  • Masuoka Y; Insect Design Technology Group, Division of Insect Advanced Technology, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), 1-2 Owashi, Tsukuba, Ibaraki, 305-8634, Japan.
  • Jouraku A; Research Center for Agricultural Information Technology (RCAIT), National Agriculture and Food Research Organization (NARO), 1-31-1 Kannondai, Tsukuba, Ibaraki, 305-0856, Japan.
  • Tsubota T; Insect Design Technology Group, Division of Insect Advanced Technology, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), 1-2 Owashi, Tsukuba, Ibaraki, 305-8634, Japan.
  • Ono H; Silkworm Research Group, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), 1-2 Owashi, Tsukuba, Ibaraki, 305-8634, Japan.
  • Chiba H; Database Center for Life Science (DBCLS), Joint Support-Center for Data Science Research, Research Organization of Information and Systems, 178-4-4 Wakashiba, Kashiwa, Chiba, 277-0871, Japan.
  • Sezutsu H; Laboratory of BioDX, PtBio Co-Creation Research Center, Genome Editing Innovation Center, Hiroshima University, 3-10-23 Kagamiyama, Higashi-Hiroshima city, Hiroshima, 739-0046, Japan.
  • Bono H; PtBio Inc., 3-10-23 Kagamiyama, Higashi-Hiroshima city, Hiroshima, 739-0046, Japan.
  • Yokoi K; Database Center for Life Science (DBCLS), Joint Support-Center for Data Science Research, Research Organization of Information and Systems, 178-4-4 Wakashiba, Kashiwa, Chiba, 277-0871, Japan.
Sci Data ; 11(1): 709, 2024 Jun 28.
Article in En | MEDLINE | ID: mdl-38942767
ABSTRACT
Time-course transcriptome expression data were constructed for four parts of the silk gland (anterior, middle, and posterior parts of the middle silk gland, along with the posterior silk gland) in the domestic silkworm, Bombyx mori, from days 0 to 7 of the last-instar larvae. For sample preparation, silk glands were extracted from one female and one male larva every 24 hours accurately after the fourth ecdysis. The reliability of these transcriptome data was confirmed by comparing the transcripts per million (TPM) values of the silk gene and quantitative reverse transcription PCR results. Hierarchical cluster analysis results supported the reliability of transcriptome data. These data are likely to contribute to the progress in molecular biology and genetic research using B. mori, such as elucidating the mechanism underlying the massive production of silk proteins, conducting entomological research using a meta-analysis as a model for lepidopteran insect species, and exploring medical research using B. mori as a model for disease species by utilising transcriptome data.
Subject(s)

Full text: 1 Database: MEDLINE Main subject: Bombyx / Transcriptome / Larva Limits: Animals Language: En Year: 2024 Type: Article

Full text: 1 Database: MEDLINE Main subject: Bombyx / Transcriptome / Larva Limits: Animals Language: En Year: 2024 Type: Article