Genetic diversity of astroviruses detected in wild aquatic birds in Hong Kong.
Virol J
; 21(1): 153, 2024 Jul 07.
Article
in En
| MEDLINE
| ID: mdl-38972989
ABSTRACT
Wild waterfowl serve as a reservoir of some astroviruses. Fecal samples from wild waterfowl collected at Hong Kong's Marshes were tested using pan-astrovirus reverse transcription-PCR. Positive samples underwent subsequent host identification using DNA barcoding. Based on deduced partial sequences, noteworthy samples from three astrovirus groups (mammalian, avian and unclassified astroviruses) were further analyzed by next-generation sequencing. One sample of Avastrovirus 4 clade, MP22-196, had a nearly complete genome identified. The results of ORF2 phylogenetic analysis and genetic distance analysis indicate that Avastrovirus 4 is classified as a distinct subclade within Avastrovirus. MP22-196 has typical astrovirus genome characteristics. The unique characteristics and potential differences of this genome, compared to other avian astrovirus sequences, involve the identification of a modified sgRNA sequence situated near the ORF2 start codon, which precedes the ORF1b stop codon. Additionally, the 3' UTR of MP22-196 is shorter than other avian astroviruses. This study expands our understanding of the Avastrovirus 4 clade.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Phylogeny
/
Genetic Variation
/
Birds
/
Genome, Viral
/
Astroviridae Infections
/
Feces
Limits:
Animals
Country/Region as subject:
Asia
Language:
En
Year:
2024
Type:
Article