A micro solid-phase radioimmunoassay for detection of herpesvirus type-specific antibody: specificity and sensitivity.
J Virol Methods
; 6(2): 85-97, 1983 Feb.
Article
in En
| MEDLINE
| ID: mdl-6189849
ABSTRACT
The specificity and sensitivity of a micro solid-phase radioimmunoassay (micro-SPRIA) that detects type-specific IgG antibody to herpes simplex virus types 1 and 2 (HSV1 and HSV2) were evaluated. Glycoproteins VP123 (molecular weight, 123,000) of HSV1 and VP119 (molecular weight, 119,000) of HSV2 were found to display the greatest degree of antigenic type-specificity of several HSV antigens tested with the micro-SPRIA technique. When testing a group of sera, negative for anti-HSV antibodies by microneutralization, in the micro-SPRIA, a range of negative reactivities was noted, suggesting that cut-points should be determined for each antigen preparation. The micro-SPRIA detected appropriate antibody activity in patients with recurrent infection and a marked agreement was noted in comparison to detection of anti-HSV antibodies measured with the microneutralization test. The type-specificity of the micro-SPRIA was substantiated by the independence of test results using VP119 and VP123 antigens for a random group of positive sera. The assay is rapid, specific, and sensitive and allows the testing of multiple serum samples with a standardized set of reagents.
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Database:
MEDLINE
Main subject:
Radioimmunoassay
/
Simplexvirus
/
Herpes Simplex
/
Antibodies, Viral
Type of study:
Diagnostic_studies
/
Evaluation_studies
Limits:
Humans
Language:
En
Year:
1983
Type:
Article