A monoclonal antibody to the double-stranded polyribonucleotide complex poly(A) X poly(U).
Mol Immunol
; 21(10): 939-44, 1984 Oct.
Article
in En
| MEDLINE
| ID: mdl-6504052
ABSTRACT
A monoclonal antibody to the double-stranded polyribonucleotide complex poly(A) . poly(U) was derived from the fusion of spleen cells from immunized DBA/2 mice and the P3 X X63-Ag8 plasma cytoma. Specificity studies using radioimmunoassays showed that the anti-poly(A) . poly(U) does not cross-react with single-stranded polyribonucleotides. RNA X DNA hybrids or DNAs. In addition to RNA duplexes associating adenine and uracil, it recognizes synthetic poly(I) . poly(C) and naturally occurring reovirus RNA. It is thus directed against a conformational epitope with an absolute requirement for two polyribose phosphate chains. However, the antibody does not cross-react with poly(G) . poly(C) and is therefore able to distinguish between RNA double helices.
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Database:
MEDLINE
Main subject:
Poly A-U
/
Antibodies, Monoclonal
Limits:
Animals
Language:
En
Year:
1984
Type:
Article