Monoclonal antibodies to choline acetyltransferase of rat brain.
FEBS Lett
; 155(2): 306-10, 1983 May 08.
Article
in En
| MEDLINE
| ID: mdl-6852242
ABSTRACT
Monoclonal antibodies to rat brain choline acetyltransferase were produced by the hybridoma technique. Two stable cell lines, Ab-57 and Ab-60, secreted immunoglobulin of subclass IgG1. The monoclonal antibodies bound to choline acetyltransferase without blocking catalytic activity. Affinity of Ab-57 was 100-times higher than that of Ab-60. Both antibodies bound to the rat enzyme in a mutually exclusive fashion. The antibodies showed cross-species reactivity with choline acetyltransferase from several mammalian brains.
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Database:
MEDLINE
Main subject:
Brain
/
Choline O-Acetyltransferase
/
Antibodies, Monoclonal
Limits:
Animals
Language:
En
Year:
1983
Type:
Article