Lymphokine production in primary mixed lymphocyte reactions. I. Characteristics of responding and stimulating cells.
Exp Cell Biol
; 48(3): 180-90, 1980.
Article
in En
| MEDLINE
| ID: mdl-6989664
ABSTRACT
The characteristics of the responder and stimulating cells involved in migration inhibition factor (MIF) production in primary 'one-way' mouse mixed lymphocyte reactions (MLR) were analyzed by using an indirect agarose droplet assay. T-lymphocytes are mainly responsible for MIF release, as shown by pretreatment with anti-Thy 1.2 serum plus complement or purification over a nylon wool column. On the other hand, macrophages and B-lymphocytes appear to be optimal stimulating cells. T-lymphocytes as stimulating cells induce MIF release, but to a much lesser degree than macrophages and B-lymphocytes. The kinetics of MIF production in MLR is related to the kind of stimulating cells employed. Lastly, the ability to release MIF is already present in the spleen of 1- to 2-week-old mice, lasts until 20 weeks of age and declines to undetectable levels at 50 weeks of age.
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Database:
MEDLINE
Main subject:
T-Lymphocytes
/
Leukocyte Migration-Inhibitory Factors
/
Lymphokines
Limits:
Animals
Language:
En
Year:
1980
Type:
Article