Respiratory syncytial virus nucleocapsid protein (N) expressed in insect cells forms nucleocapsid-like structures.
Virus Res
; 31(2): 187-201, 1994 Feb.
Article
in En
| MEDLINE
| ID: mdl-7513931
ABSTRACT
The gene coding for the N protein of RSV strain Long has been cloned and sequenced. It was introduced behind the polyhedrin promoter of the shuttle vector pVL941 and baculoviruses containing the N gene were constructed by homologous recombination. Infection of Spodoptera frugiperda 9 cells resulted in the production of large amounts of a protein similar in size and antigenicity to the authentic N protein. The baculovirus expressed N protein was concentrated in the cytoplasm of the insect cells and could be extracted at low salt concentration. Nucleocapsid structures similar to those purified from RSV-infected cells could be observed by electron microscopy after negative staining of cellular extracts.
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Database:
MEDLINE
Main subject:
Respiratory Syncytial Viruses
/
Viral Core Proteins
/
Capsid
/
Genes, Viral
Limits:
Animals
Language:
En
Year:
1994
Type:
Article