Mutagenesis by metal-induced oxygen radicals.
Environ Health Perspect
; 102 Suppl 3: 57-61, 1994 Sep.
Article
in En
| MEDLINE
| ID: mdl-7843138
ABSTRACT
To assess the contribution of reactive oxygen species (ROS) to metal-induced mutagenesis, we have determined the spectrum of mutations in the lacZ alpha gene after exposure of M13mp2 DNA to Fe2+, Cu2+, and Ni2+. With iron and copper ions, mutations are clustered and are predominantly single-base substitutions. Fe, Cu, and phorbol ester-stimulated neutrophils also produced tandem double CC-->TT mutations. This mutation may provide a marker for the role of oxidative damage in carcinogenesis. Mutagenesis by Ni2+ required the complexing of the metal to a tripeptide and the addition of H2O2. To assess the contribution of ROS in mammalian cells, we determined the spectrum of mutations produced when purified DNA polymerases-alpha and -beta synthesized DNA using a template that had been damaged by ROS. The mutation spectra produced by the two polymerases indicates that these enzymes substitute different nucleotides opposite the same lesions.
Full text:
1
Database:
MEDLINE
Main subject:
Reactive Oxygen Species
/
Copper
/
Iron
/
Mutagens
/
Nickel
Limits:
Humans
Language:
En
Year:
1994
Type:
Article