The sex ratio of normal and manipulated human sperm quantitated by the polymerase chain reaction.
Fertil Steril
; 59(2): 387-92, 1993 Feb.
Article
in En
| MEDLINE
| ID: mdl-8425636
ABSTRACT
OBJECTIVE:
To establish the primary sex ratio, the relative abundance of X and Y chromosome-bearing sperm, in unselected sperm and in sperm selected by swim-up or Sephadex filtration (SpermPrep column; Fertility Technologies, Inc., Natick, MA). This was done to evaluate the possibility that these semen manipulations change the primary sex ratio.DESIGN:
Ninety-eight unmanipulated semen samples were analyzed for sex chromosome content using quantitative polymerase chain reaction (PCR). A smaller number of samples was analyzed before and after either swim-up or Sephadex filtration.RESULTS:
The mean percentage of all sex chromosomes identified as a Y chromosome in unmanipulated semen samples ranged from 41.9% to 56.7%, with a mean average of 50.3%. There was no significant change in sex chromosome composition after either swim-up (n = 17) or column filtration (n = 20).CONCLUSIONS:
The chromosome compositions of semen samples from a large number of men have equal numbers of X and Y. Swim-up and SpermPrep filtration do not appear to alter the primary sex ratio.
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Database:
MEDLINE
Main subject:
Sex Ratio
/
Spermatozoa
/
Polymerase Chain Reaction
/
Reproductive Techniques
Limits:
Humans
/
Male
Language:
En
Year:
1993
Type:
Article