Human growth hormone receptor: cloning and expression of the full-length complementary DNA after site-directed inactivation of a cryptic bacterial promoter.

Bieth, E; Cahoreau, C; Cholin, S; Molinas, C; Rochiccioli, P; Devauchelle, G; Tauber, M

Bieth, E; Cahoreau, C; Cholin, S; Molinas, C; Rochiccioli, P; Devauchelle, G; Tauber, M.

Affiliation

Bieth E; Centre de Recherche sur la Croissance, Pavillon Leriche, Centre Hospitalier Universitaire de Purpan, Toulouse, France.

Gene ; 194(1): 97-105, 1997 Jul 18.

Article in En | MEDLINE | ID: mdl-9266678

Subject(s)

Escherichia coli/genetics; Promoter Regions, Genetic; Receptors, Somatotropin/biosynthesis; Receptors, Somatotropin/genetics; Recombinant Proteins/biosynthesis; Animals; Baculoviridae; Base Sequence; Cloning, Molecular; Consensus Sequence; DNA Primers; DNA, Complementary/biosynthesis; Escherichia coli/growth & development; Glycosylation; Human Growth Hormone/metabolism; Humans; Kinetics; Mutagenesis, Site-Directed; Polymerase Chain Reaction; Receptors, Somatotropin/metabolism; Recombinant Proteins/metabolism; Restriction Mapping; Spodoptera; Transfection

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Database: MEDLINE Main subject: Receptors, Somatotropin / Recombinant Proteins / Promoter Regions, Genetic / Escherichia coli Limits: Animals / Humans Language: En Year: 1997 Type: Article

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