Expression and purification of soluble, active heterodimeric guanylyl cyclase from baculovirus.

Gupta, G; Kim, J; Yang, L; Sturley, S L; Danziger, R S.

Affiliation

Gupta G; Columbia University, College of Physicians & Surgeons, New York, New York 10032, USA.

Protein Expr Purif ; 10(3): 325-30, 1997 Aug.

Article in En | MEDLINE | ID: mdl-9268679

Subject(s)

Guanylate Cyclase/isolation & purification; Animals; Baculoviridae/genetics; Blotting, Western; Cell Line; Chromatography, Affinity; Dimerization; Electrophoresis, Polyacrylamide Gel; Genetic Vectors; Guanylate Cyclase/biosynthesis; Guanylate Cyclase/genetics; Guanylate Cyclase/metabolism; Histidine/genetics; Isoenzymes/isolation & purification; Protein Binding; Protein Conformation; Protoporphyrins/pharmacology; Recombinant Proteins/biosynthesis; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Spectrophotometry; Spodoptera

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Database: MEDLINE Main subject: Guanylate Cyclase Limits: Animals Language: En Year: 1997 Type: Article

Database: MEDLINE Main subject: Guanylate Cyclase Limits: Animals Language: En Year: 1997 Type: Article