Development and validation of liquid chromatography and capillary electrophoresis methods for acarbose determination in pharmaceutical tablets.

ABSTRACT

Liquid chromatography and capillary zone electrophoresis, respectively coupled to an evaporative light scattering detector and a UV detector have been developed for the analysis of acarbose without any derivatization procedure. The electrophoretic separation of acarbose anomers was achieved through the manipulation of the working temperature. Both methods were validated and showed good validation data in terms of precision, accuracy and linearity. The validated methods were successfully applied to the dosage of acarbose in commercially available Glucobay tablets.