An investigation on in vitro and in vivo antimicrobial properties of the antidepressant: amitriptyline hydrochloride
Braz. j. microbiol
; 41(3): 635-642, Oct. 2010. graf, tab
Article
en En
| LILACS
| ID: lil-549405
Biblioteca responsable:
BR32.1
ABSTRACT
The antidepressant drug amitriptyline hydrochloride was obtained in a dry powder form and was screened against 253 strains of bacteria which included 72 Gram positive and 181 Gram negative bacteria and against 5 fungal strains. The minimum inhibitory concentration (MIC) was determined by inoculating a loopful of an overnight peptone water culture of the organism on nutrient agar plates containing increasing concentrations of amitriptyline hydrochloride (0, 10 µg/mL, 25 µg/mL, 50 µg/mL, 100 µg/mL, 200 µg/mL). Amitriptyline hydrochloride exhibited significant action against both Gram positive and Gram negative bacteria at 25-200 µg/mL. In the in vivo studies it was seen that amitriptyline hydrochloride at a concentration of 25 µg/g and 30 µg/g body weight of mouse offered significant protection to Swiss strain of white mice when challenged with 50 median lethal dose (MLD) of a virulent strain of Salmonella typhimurium NCTC 74. The in vivo data were highly significant (p<0.001) according to the chi-square test.
Palabras clave
Texto completo:
1
Banco de datos:
LILACS
Asunto principal:
Infecciones Bacterianas
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Técnicas In Vitro
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Farmacorresistencia Microbiana
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Reacción en Cadena de la Polimerasa
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Amitriptilina
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Antibacterianos
Límite:
Animals
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Humans
Idioma:
En
Año:
2010
Tipo del documento:
Article