Structure, tissue distribution, and pharmacological characterization of Xenopus orexins.
Peptides
; 20(10): 1169-76, 1999.
Article
en En
| MEDLINE
| ID: mdl-10573288
ABSTRACT
We isolated the Xenopus gene encoding prepro-orexin to predict the structures of orexins in submammalian chordates. Putative mature Xenopus orexin-A and -B are highly similar to each mammalian counterpart. Especially, the C-terminal 10 residues were highly conserved among these species and isopeptides. Immunohistochemical examination of Xenopus brain revealed that orexin-containing neurons were highly specifically localized in the ventral hypothalamic nucleus. A rich network of immunoreactive fibers was found in various regions of the Xenopus brain. The distribution was similar to that of mammalian orexins. Xenopus orexin-A and -B specifically bind and activate human orexin receptors expressed in Chinese hamster ovary cells. Of interest, Xenopus orexin-B had several-fold higher affinity to human OX2R compared with human orexins. These results suggest that Xenopus orexin-B might be a useful pharmacological tool as an OX2R selective high-affinity agonist.
Buscar en Google
Banco de datos:
MEDLINE
Asunto principal:
Neuropéptidos
/
Proteínas Portadoras
/
Péptidos y Proteínas de Señalización Intracelular
Límite:
Animals
/
Humans
Idioma:
En
Año:
1999
Tipo del documento:
Article