Voltage- and gamma-aminobutyric acid-activated membrane currents in the human medulloblastoma cell line MHH-MED-3.
Neurosci Lett
; 287(1): 53-6, 2000 Jun 16.
Article
en En
| MEDLINE
| ID: mdl-10841989
ABSTRACT
The whole-cell patch clamp technique was used to characterize voltage- and neurotransmitter-activated currents in the medulloblastoma cell line MHH-MED-3 and cells from tissue slices and primary cultures of two medulloblastoma biopsies. These preparations revealed similar electrophysiological properties. All tested cells displayed 4-aminopyridine-sensitive delayed rectifying K(+) currents, gamma-aminobutyric acid(A) receptor-mediated Cl(-) currents and most of them inward rectifier K(+) currents. Transient inward currents were mainly carried by low-voltage activated T-type Ca(2+) channels in MHH-MED-3 cells, and tetrodotoxin-sensitive Na(+) channels in cells from the primary culture. From these characteristics we conclude that medulloblastoma cells share physiological features with developing cerebellar granule cells at an immature stage.
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Banco de datos:
MEDLINE
Asunto principal:
Células Tumorales Cultivadas
/
Membrana Celular
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Neoplasias Cerebelosas
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Ácido gamma-Aminobutírico
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Canales Iónicos
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Meduloblastoma
Límite:
Adolescent
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Humans
/
Male
Idioma:
En
Año:
2000
Tipo del documento:
Article