[Standardization and evaluation of the modified vibriocidal assay]. / Estandarización y evaluación del ensayo vibriocida modificado.
Rev Cubana Med Trop
; 51(3): 156-9, 1999.
Article
en Es
| MEDLINE
| ID: mdl-10887580
ABSTRACT
The vibriocidal antibody test is a reliable and well-documented method to determine bacterial antibodies to Vibrio cholerae 01 antigens. It consists of mixing serum dilutions and a steady quantity of bacteria and supplement to cause cell lysis. Titer is determined by visual observation. In this paper, we implemented a change in the presented method where a pH and glucose indicator was added to the culture medium used to stop the reaction, which allowed a quicker reading by any person who are not very familiar with the carrying out of this test since the colour change in the plaque is quite evident.
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Banco de datos:
MEDLINE
Asunto principal:
Vibrio cholerae
/
Actividad Bactericida de la Sangre
Límite:
Animals
/
Humans
Idioma:
Es
Año:
1999
Tipo del documento:
Article