Serum and mucosal antibodies of infected foals recognized two distinct epitopes of VapA of Rhodococcus equi.
FEMS Immunol Med Microbiol
; 34(4): 299-306, 2002 Dec 13.
Article
en En
| MEDLINE
| ID: mdl-12443830
ABSTRACT
Virulence-associated protein A (VapA) of Rhodococcus equi has been proposed for use both as a vaccine and as a target for antibodies in immunotherapy and diagnostic tests. Epitope mapping of VapA allowed the identification of two B cell epitopes associated with R. equi pneumonia. The peptide NLQKDEPGRASDT was confirmed as an immunodominant N-terminal B cell epitope recognized by all sera from infected foals while VSFQYNAVGPYLNINFFDSS (C-terminal B cell epitope) was exclusively recognized by IgA from the tracheal aspirates. Moreover, specific antibodies produced against the VapA-specific peptide reacted with a major protein (approximately 20 kDa) from R. equi antigens separated by two-dimensional gel electrophoresis. The strong reactivity of mucosal IgA from infected foals with the conserved peptides might constitute an attractive target for diagnosis and vaccine.
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Banco de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Infecciones por Actinomycetales
/
Inmunoglobulina A Secretora
/
Glicoproteínas de Membrana
/
Rhodococcus equi
/
Mapeo Epitopo
/
Factores de Virulencia
/
Enfermedades de los Caballos
/
Anticuerpos Antivirales
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Año:
2002
Tipo del documento:
Article