Simultaneous determination of E-2-nonenal and beta-damascenone in beer by reversed-phase liquid chromatography with UV detection.
J Chromatogr A
; 1032(1-2): 17-22, 2004 Apr 02.
Article
en En
| MEDLINE
| ID: mdl-15065771
ABSTRACT
A method for the simultaneous determination of E-2-nonenal and beta-damascenone in beer by reversed-phase liquid chromatography using UV detection is presented. The method consists of beer steam distillation, followed by an extraction/concentration step using Sep-Pak Plus C18 RP cartridges and determination by HPLC at 226 nm UV-absorption maximum. The identity of the compounds was confirmed by GC analysis with MS detection of the isolated fractions. A recovery factor of approximately 80% was obtained for beta-damascenone with a R.S.D. of 3%. E-2-Nonenal and beta-damascenone were monitored in a comparative study of fresh and either naturally and forced aged beer. The results obtained show that both compounds have a similar behaviour through an extended storage of beer and consequently can be used as good analytical markers of beer ageing. Nevertheless, the use of beta-damascenone seems to be more convenient because this compound appears in beer in higher concentrations than E-2-nonenal, thus making it easier to measure.
Buscar en Google
Banco de datos:
MEDLINE
Asunto principal:
Espectrofotometría Ultravioleta
/
Cerveza
/
Cromatografía Líquida de Alta Presión
/
Norisoprenoides
/
Aldehídos
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Año:
2004
Tipo del documento:
Article