Electroimmobilization of proinsulin C-peptide to a quartz crystal microbalance sensor chip for protein affinity purification.
Anal Biochem
; 341(1): 89-93, 2005 Jun 01.
Article
en En
| MEDLINE
| ID: mdl-15866532
ABSTRACT
Proinsulin C-peptide was electroimmobilized to a quartz crystal microbalance sensor chip, localizing this low-pI peptide for covalent attachment to activated surface carboxyl groups. The resulting chip was used in a continuous flow biosensor to capture anti-C-peptide antibodies, which could subsequently be eluted in 5% formic acid between air bubbles for efficient recovery and mass spectrometric identification. The method is reproducible through repeated cycles, providing affinity purification of proteins under real-time monitoring of the binding and elution processes.
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Banco de datos:
MEDLINE
Asunto principal:
Proinsulina
/
Cuarzo
/
Péptido C
/
Técnicas Biosensibles
Límite:
Humans
Idioma:
En
Año:
2005
Tipo del documento:
Article