Testing of amplified fragment length polymorphism (AFLP) technique as a tool for molecular epidemiology of Trichinella nativa.
Vet Parasitol
; 132(1-2): 19-22, 2005 Sep 05.
Article
en En
| MEDLINE
| ID: mdl-15985333
ABSTRACT
A total of nine Trichinella nativa isolates were compared by amplified fragment length polymorphism (AFLP). Four hundred nanograms of genomic DNA from a pool of 10--20 larvae were digested using HindIII and MseI restriction endonucleases. Of the 16 primer combinations initially tested, Hind-C and Mse-C primers showed rich polymorphism with approximately 40--90 bands in the range of 30--270 bp. Genetic similarities were estimated visually. AFLP provided discriminatory banding patterns and may therefore be used as a method for detecting variation in T. nativa populations. However, the heterogeneous patterns obtained from pooled samples emphasize the need for further development of the sampling and numerical analysis of the patterns for epidemiological and taxonomical interpretation.
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Banco de datos:
MEDLINE
Asunto principal:
Trichinella
/
Técnica del ADN Polimorfo Amplificado Aleatorio
Tipo de estudio:
Screening_studies
Límite:
Animals
Idioma:
En
Año:
2005
Tipo del documento:
Article