ERK/MAPK regulates ecdysteroid and sorbitol metabolism for embryonic diapause termination in the silkworm, Bombyx mori.
J Insect Physiol
; 52(6): 569-75, 2006 Jun.
Article
en En
| MEDLINE
| ID: mdl-16546206
ABSTRACT
The eggs of the silkworm Bombyx mori undergo a state of suspended overt development and diminished metabolism called "diapause" to escape adverse environmental conditions. Termination of Bombyx embryonic diapause requires 2-3 months of low temperature (5 degrees C), but the molecular mechanisms underlying diapause termination are unknown. Diapause termination requires a decrease in the sorbitol concentration, which arrests embryonic development, and the secretion factors from yolk cells that promote embryonic development. In the present study, we report that 20-hydroxyecdysone promoted the development of denuded embryos and that ecdysteroid-phosphate phosphatase (EPPase), which is a key enzyme for active ecdysteroid production, was induced by incubation of diapausing eggs at 5 degrees C. In dechorionated egg cultures, extracellular signal-regulated kinase (ERK), which is activated by incubating diapausing eggs at 5 degrees C, regulated sorbitol-glycogen conversion, ecdysteroid secretion via gene transcription of key enzymes, sorbitol dehydrogenase-2, and EPPase, suggesting that ERK has a key role in diapause termination.
Buscar en Google
Banco de datos:
MEDLINE
Asunto principal:
Bombyx
/
Sorbitol
/
Ecdisteroides
/
Quinasas MAP Reguladas por Señal Extracelular
/
Desarrollo Embrionario
Límite:
Animals
Idioma:
En
Año:
2006
Tipo del documento:
Article