Efficient and non-denaturing membrane solubilization combined with enrichment of membrane protein complexes by detergent/polymer aqueous two-phase partitioning for proteome analysis.
J Chromatogr A
; 1122(1-2): 35-46, 2006 Jul 28.
Article
en En
| MEDLINE
| ID: mdl-16682048
ABSTRACT
It is of central interest in membrane proteomics to establish methods that combine efficient solubilization with enrichment of proteins and intact protein complexes. We have investigated the quantitative and qualitative solubilization efficiency of five commercially available detergents using mitochondria from the yeast Saccharomyces cerevisiae as model system. Combining the zwitterionic detergent Zwittergent 3-10 and the non-ionic detergent Triton X-114 resulted in a complementary solubilization of proteins, which was similar to that of the anionic detergent sodium dodecyl sulfate (SDS). The subsequent removal of soluble proteins by detergent/polymer two-phase system partitioning was further enhanced by addition of SDS and increasing pH. A large number of both integral and peripheral membrane protein subunits from mitochondrial membrane protein complexes were identified in the detergent phase. We suggest that the optimized solubilization protocol in combination with detergent/polymer two-phase partitioning is a mild and efficient method for initial enrichment of membrane proteins and membrane protein complexes in proteomic studies.
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Banco de datos:
MEDLINE
Asunto principal:
Polímeros
/
Membrana Celular
/
Proteoma
/
Detergentes
/
Proteínas de la Membrana
Tipo de estudio:
Prognostic_studies
/
Qualitative_research
Idioma:
En
Año:
2006
Tipo del documento:
Article