Daxx represses expression of a subset of antiapoptotic genes regulated by nuclear factor-kappaB.
Cancer Res
; 66(18): 9026-35, 2006 Sep 15.
Article
en En
| MEDLINE
| ID: mdl-16982744
ABSTRACT
Daxx is a nuclear protein that localizes to PML oncogenic domains, sensitizes cells to apoptosis, and functions as a transcriptional repressor. We found that Daxx represses the expression of several antiapoptotic genes regulated by nuclear factor-kappaB, including cIAP2, in human tumor cell lines. Daxx interacts with RelB and inhibits RelB-mediated transcriptional activation of the human cIAP2 gene promoter. Daxx also forms complexes with RelB while bound to its target sites in the cIAP2 promoter, as shown by electrophoretic mobility shift assays and chromatin immunoprecipitation experiments. Using cells from daxx-/- mouse embryos, we observed that levels of the corresponding murine c-IAP mRNA and protein are increased in cells lacking Daxx. Conversely, c-IAP mRNA and protein levels were reduced in relB-/- cells. Taken together, these observations provide a mechanism that links two previously ascribed functions of Daxx transcriptional repression and sensitization to apoptosis.
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Banco de datos:
MEDLINE
Asunto principal:
Proteínas Nucleares
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Regulación Neoplásica de la Expresión Génica
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FN-kappa B
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Proteínas Adaptadoras Transductoras de Señales
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Proteínas Inhibidoras de la Apoptosis
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Factor de Transcripción ReIB
Límite:
Animals
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Humans
Idioma:
En
Año:
2006
Tipo del documento:
Article