Mapping of a retinoic acid-responsive element in the promoter region of the complement factor H gene.
J Biol Chem
; 265(33): 20065-8, 1990 Nov 25.
Article
en En
| MEDLINE
| ID: mdl-1700780
ABSTRACT
Retinoic acid receptors are members of the steroid/thyroid hormone receptor superfamily. Pursuant to the discovery that dexamethasone increases complement factor H expression, we examined the effects of retinoic acid on this gene. Both H mRNA and protein levels are increased by retinoic acid in L cells. Using the luciferase reporter gene system we have identified a region of the H promoter required for the retinoic acid response. This region contains an imperfect palindrome of the TGACC motif, present in thyroid hormone and estrogen-responsive elements. We demonstrate specific binding of the retinoic acid receptor beta to this sequence of the H gene by DNA-protein gel retardation assay. Therefore, these studies extend the sphere of influence of the retinoids to complement, an intrinsic component of the humoral immune system.
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Banco de datos:
MEDLINE
Asunto principal:
Transcripción Genética
/
Tretinoina
/
Proteínas Inactivadoras del Complemento C3b
/
Regiones Promotoras Genéticas
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Año:
1990
Tipo del documento:
Article