Biological role of estrogen receptor beta in salivary gland adenocarcinoma cells.
Clin Cancer Res
; 12(20 Pt 1): 5994-9, 2006 Oct 15.
Article
en En
| MEDLINE
| ID: mdl-17062671
ABSTRACT
PURPOSE:
This study is intended to investigate the biological role of estrogen receptor (ER) nongenomic signaling in salivary gland adenocarcinoma cells that predominantly express ERbeta. EXPERIMENTALDESIGN:
Salivary gland adenocarcinoma cell lines HSG and HSY were used to study the effect of diarylpropionitrile and estrogen on the nongenomic signaling of ERbeta, cytoskeletal remodeling, and cell motility.RESULTS:
We found that diarylpropionitrile and estrogen triggered rapid activation of the extracellular signal-regulated kinase 1/2 (ERK), Src, and focal adhesion kinase signaling pathways. Estrogen stimulation also induced long cytoplasmic extensions, filopodia formation, and abnormal outgrowths in both HSG and HSY cells. We further observed that ligand-induced migration of these cells was blocked by the pure antiestrogen ICI 182780 and the mitogen-activated protein/ERK kinase inhibitor PD98059, indicating that estrogen-induced cell migration is mediated by the activation of ERbeta nongenomic signaling.CONCLUSION:
These results clearly showed that ERbeta nongenomic signaling is active in salivary gland cells and has a biological role in migration, presumably via the stimulation of ERK1/2. In future, the findings of this study might have clinical importance as several ERbeta-selective agonists are currently being available, and these could potentially be used for therapeutic targeting of ERbeta-positive salivary tumors.
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Banco de datos:
MEDLINE
Asunto principal:
Neoplasias de las Glándulas Salivales
/
Adenocarcinoma
/
Receptor beta de Estrógeno
Límite:
Humans
Idioma:
En
Año:
2006
Tipo del documento:
Article