Construction of a binding site for human immunodeficiency virus type 1 gp120 in rat CD4.
J Exp Med
; 175(1): 301-4, 1992 Jan 01.
Article
en En
| MEDLINE
| ID: mdl-1730924
ABSTRACT
The human immunodeficiency virus (HIV-1) infects T lymphocytes via an interaction between the virus envelope glycoprotein gp120 and the CD4 antigen of T helper cells. Previous studies demonstrated that mutations in various regions of CD4 domain 1 lead to the loss of gp120 binding. In the present study the gp120 binding site was constructed in rat CD4 by replacing rat with human CD4 sequence. A series of mutants was constructed the best of which bound gp120 with an affinity only twofold less than that of human CD4. The data indicate that the gp120 binding site of human CD4 is constituted by residues 33-58 of domain 1.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Antígenos CD4
/
Proteína gp120 de Envoltorio del VIH
/
VIH-1
Límite:
Animals
Idioma:
En
Año:
1992
Tipo del documento:
Article