Genetic analysis of high-metastatic clone of RCT sarcoma in mice, and its growth regression in vivo in response to angiogenesis inhibitor TNP-470.
J Exp Clin Cancer Res
; 26(1): 101-7, 2007 Mar.
Article
en En
| MEDLINE
| ID: mdl-17550138
ABSTRACT
Genetic analysis of a high-metastatic clone of RCT sarcoma (HM-RCT) was the aim of the study. HM-RCT was developed by the lung passage as well as limiting dilution method from the original RCT sarcoma, in which a tumor was spontaneously developed in a C3H/He mouse. HM-RCT expressed enhanced POU domain (class 2, associating factor 1), adenylate cyclase 7, procollagen type III (alpha), A kinase anchor protein 4 and Ehm (expressed on high-metastatic cells) and 11 expressed sequence tags (ESTs). compared with the original clone of RCT. Eighteen specific genes and 14 ESTs were underexpressed in HM-RCT. We investigated the effects of angiogenesis inhibitor TNP-470 on tumor growth and metastasis of this HM-RCT in vivo. In an experimental group, mice received TNP-470 (30 mg/kg) intraperitoneally every other day. After 5 weeks, the growth of the TNP-470-treated tumor was significantly suppressed in vivo, but did not affect the metastasis. The proportion of positive PCNA-stained cells and cellular telomerase activity was significantly low in response to TNP-470.
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Banco de datos:
MEDLINE
Asunto principal:
Sarcoma Experimental
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Sesquiterpenos
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Regulación Neoplásica de la Expresión Génica
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Inhibidores de la Angiogénesis
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Ciclohexanos
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Neoplasias Pulmonares
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Neovascularización Patológica
Tipo de estudio:
Prognostic_studies
Idioma:
En
Año:
2007
Tipo del documento:
Article