Repertoires of aggregation-resistant human antibody domains.
Protein Eng Des Sel
; 20(8): 413-6, 2007 Aug.
Article
en En
| MEDLINE
| ID: mdl-17720749
ABSTRACT
We recently described a method for the generation of a large human domain antibody repertoire involving combinatorial assembly of CDR building blocks from a smaller repertoire comprising a high frequency of aggregation-resistant antibody domains. Here we show that the frequency of aggregation-resistant domains in the combinatorial repertoire remained high. Furthermore, one of the antigen-binding domains selected from the combinatorial repertoire retained its binding properties through 25 cycles of thermal denaturation, suggesting that antibody domains can be created that rival the heat-resistance of thermophilic proteins such as Taq polymerase.
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Banco de datos:
MEDLINE
Asunto principal:
Cadenas Pesadas de Inmunoglobulina
/
Anticuerpos
Límite:
Humans
Idioma:
En
Año:
2007
Tipo del documento:
Article