Characterization of a mammalian cDNA for an inactivating voltage-sensitive K+ channel.
Neuron
; 7(3): 471-83, 1991 Sep.
Article
en En
| MEDLINE
| ID: mdl-1840649
ABSTRACT
A cDNA clone encoding a K+ channel polypeptide with 72% amino acid sequence identity to Drosophila Shal was isolated from rat hippocampus. Functional expression of the cDNA in Xenopus oocytes generated 4-amino-pyridine-sensitive K+ channels displaying rapid inactivation kinetics. The fastest component of inactivation was slowed by the deletion of 3 basic residues in the amino-terminal region. Northern blots revealed that the mRNA encoding this K+ channel polypeptide was expressed at a similar level in the brain and in the heart. In situ hybridization revealed that the mRNA encoding this K+ channel appeared concentrated in the hippocampus, dentate gyrus, and habenular nucleus in the brain. Thus, this K+ channel polypeptide is likely to form some of the A-type K+ channels expressed in the mammalian nervous system and heart.
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Banco de datos:
MEDLINE
Asunto principal:
Canales de Potasio
/
Hipocampo
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Año:
1991
Tipo del documento:
Article