Adiponectin plays a protective role in caerulein-induced acute pancreatitis in mice fed a high-fat diet.
Gut
; 57(10): 1431-40, 2008 Oct.
Article
en En
| MEDLINE
| ID: mdl-18579666
ABSTRACT
BACKGROUND:
Obesity is a risk factor for acute pancreatitis (AP), but the molecular mechanism remains unclear. Adiponectin, an adipose tissue-derived secretory factor, has anti-inflammatory properties in addition to various biological functions, and its plasma concentrations are reduced in obese subjects. However, the role of adiponectin in AP has not been investigated.AIM:
To determine the effects of adiponectin on AP.METHODS:
We investigated the effects of adiponectin on experimental AP by using adiponectin-knockout (APN-KO) mice and adenovirus-mediated adiponectin over-expression. AP was induced by 10 hourly intraperitoneal injections of low-dose caerulein (10 microg/kg) after 2 week feeding of normal chow or a high-fat diet (HFD) in wild-type (WT) and APN-KO mice. We evaluated the severity of AP biochemically and morphologically.RESULTS:
Low-dose caerulein treatment did not induce pancreatic damage in either WT or APN-KO mice under normal chow feeding. APN-KO mice, but not WT mice, fed a HFD and then treated with caerulein developed pancreatic damage and inflammation, accompanied by increased macrophage/neutrophil infiltration and upregulation of pro-inflammatory mediators such as tumour necrosis factor alpha in the pancreas. Adenovirus-mediated over-expression of adiponectin attenuated the severity of HFD/caerulein-induced AP in APN-KO mice.CONCLUSIONS:
Adiponectin plays a protective role in caerulein-induced AP in HFD-fed mice.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Pancreatitis
/
Adiponectina
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
/
Humans
Idioma:
En
Año:
2008
Tipo del documento:
Article