Ozone production by amino acids contributes to killing of bacteria.
Proc Natl Acad Sci U S A
; 105(44): 16912-7, 2008 Nov 04.
Article
en En
| MEDLINE
| ID: mdl-18971328
ABSTRACT
Reactive oxygen species produced by phagocytosing neutrophils are essential for innate host defense against invading microbes. Previous observations revealed that antibody-catalyzed ozone formation by human neutrophils contributed to the killing of bacteria. In this study, we discovered that 4 amino acids themselves were able to catalyze the production of an oxidant with the chemical signature of ozone from singlet oxygen in the water-oxidation pathway, at comparable level to antibodies. The resultant oxidant with the chemical signature of ozone exhibited significant bactericidal activity in our distinct cell-free system and in human neutrophils. The results also suggest that an oxidant with the chemical signature of ozone produced by neutrophils might potentiate a host defense system, when the host is challenged by high doses of infectious agents. Our findings provide biological insights into the killing of bacteria by neutrophils.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Ozono
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Aminoácidos
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Antibacterianos
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Neutrófilos
Límite:
Adult
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Humans
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Male
Idioma:
En
Año:
2008
Tipo del documento:
Article