Differential evaluation of excisional non-occluded wound healing in db/db mice.
Toxicol Pathol
; 37(2): 183-92, 2009 Feb.
Article
en En
| MEDLINE
| ID: mdl-19182213
ABSTRACT
The full-thickness wound in the genetically diabetic (db/db) mouse is a commonly used model of impaired wound healing. We investigated delayed healing of non-occluded, excisional, full-thickness, dermal wounds in db/db mice in comparison to their normal littermate controls and refined methods for monitoring skin wound re-epithelialization, contraction, granulation tissue formation, and inflammation. We have confirmed with a computer-assisted planimetry method the results of previous studies showing that healing of non-occluded full excision wounds in db/db mice does not occur by contraction as much as in healthy mice. In addition, we have developed separate histological methods for the assessment of re-epithelialization, contraction, granulation tissue (mature, immature, fibrosis), and inflammation (lipogranulomas, secondary, nonspecific). Using a new approach to histological assessment, we have shown that wound closure in db/db mice is delayed owing to (1) delayed granulation tissue maturation; (2) ''laced,'' widely distributed granulation tissue around fat lobules; and (3) obstruction by lipogranulomas, whereas the rate of re-epithelialization seems to be the same as in C57Bl/6 mice. This methodology should permit a more precise differentiation of effects of novel therapeutic agents on the wound healing process in db/db mice.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Piel
/
Cicatrización de Heridas
/
Heridas Penetrantes
/
Diabetes Mellitus
Tipo de estudio:
Etiology_studies
/
Evaluation_studies
Límite:
Animals
Idioma:
En
Año:
2009
Tipo del documento:
Article