GPIomics: global analysis of glycosylphosphatidylinositol-anchored molecules of Trypanosoma cruzi.
Mol Syst Biol
; 5: 261, 2009.
Article
en En
| MEDLINE
| ID: mdl-19357640
ABSTRACT
Glycosylphosphatidylinositol (GPI) anchoring is a common, relevant posttranslational modification of eukaryotic surface proteins. Here, we developed a fast, simple, and highly sensitive (high attomole-low femtomole range) method that uses liquid chromatography-tandem mass spectrometry (LC-MS(n)) for the first large-scale analysis of GPI-anchored molecules (i.e., the GPIome) of a eukaryote, Trypanosoma cruzi, the etiologic agent of Chagas disease. Our genome-wise prediction analysis revealed that approximately 12% of T. cruzi genes possibly encode GPI-anchored proteins. By analyzing the GPIome of T. cruzi insect-dwelling epimastigote stage using LC-MS(n), we identified 90 GPI species, of which 79 were novel. Moreover, we determined that mucins coded by the T. cruzi small mucin-like gene (TcSMUG S) family are the major GPI-anchored proteins expressed on the epimastigote cell surface. TcSMUG S mucin mature sequences are short (56-85 amino acids) and highly O-glycosylated, and contain few proteolytic sites, therefore, less likely susceptible to proteases of the midgut of the insect vector. We propose that our approach could be used for the high throughput GPIomic analysis of other lower and higher eukaryotes.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Trypanosoma cruzi
/
Proteínas Protozoarias
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Glicosilfosfatidilinositoles
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Glicómica
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Año:
2009
Tipo del documento:
Article