An outer arm dynein light chain acts in a conformational switch for flagellar motility.
J Cell Biol
; 186(2): 283-95, 2009 Jul 27.
Article
en En
| MEDLINE
| ID: mdl-19620633
ABSTRACT
A system distinct from the central pair-radial spoke complex was proposed to control outer arm dynein function in response to alterations in the mechanical state of the flagellum. In this study, we examine the role of a Chlamydomonas reinhardtii outer arm dynein light chain that associates with the motor domain of the gamma heavy chain (HC). We demonstrate that expression of mutant forms of LC1 yield dominant-negative effects on swimming velocity, as the flagella continually beat out of phase and stall near or at the power/recovery stroke switchpoint. Furthermore, we observed that LC1 interacts directly with tubulin in a nucleotide-independent manner and tethers this motor unit to the A-tubule of the outer doublet microtubules within the axoneme. Therefore, this dynein HC is attached to the same microtubule by two sites via both the N-terminal region and the motor domain. We propose that this gamma HC-LC1-microtubule ternary complex functions as a conformational switch to control outer arm activity.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Portadoras
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Proteínas Protozoarias
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Movimiento Celular
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Chlamydomonas reinhardtii
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Proteínas de Drosophila
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Flagelos
Límite:
Animals
Idioma:
En
Año:
2009
Tipo del documento:
Article